All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Cathepsin B Regulates the Activation of NLRP3 Inflammasome by Calcium Signaling

Author(s): Qifang Zhang, Juanjuan Duan, Bo Yang, Dejun Yu, Lijie Liu, Hua Bai

Cathepsin B (CatB) is a proteolytic enzyme present in the cytosol which catalyzes the hydrolysis of benzoyl-L-argininamide. Whether CatB can regulate the activation of NLRP3 inflammasome by affecting calcium signal is worth studying. The specific siRNA targeting CatB gene was designed. CatB gene silencing cell line (CB-si-BV2) was established in microglia BV2, and BV2 cells were treated with hydrogen peroxide. GdCl3, Cat-B inhibitor CA-074Me, calcium ion chelating agent BAPTA-AM were used to treat cells simultaneously or separately. NLRP3 protein was detected by Western Blot. NLRP3 mRNA was detected by qPCR. IL-1β and Caspase-1 were detected by ELISA. Cell viability was detected by MTT assay. Cell apoptosis was detected by Hoechst33258 staining. After treatment with hydrogen peroxide, the expression of NLRP3 protein and caspase-1 mRNA increased in BV2 cells. After adding GdCl3, the expression of NLRP3, NLRP3 mRNA, Caspase-1 and IL-1β were significantly increased. After treated with GdCl3, some NLRP3 protein was detected in CB-si-BV2 cells, NLRP3mRNA and Caspase-1 increased, compared with the control group, the difference was statistically significant. After treated with BAPTA-AM, NLRP3, NLRP3mRNA and Caspase-1 were significantly reduced. The growth rate of some CB-si-BV2 cells was significantly slowed down. There was more apoptosis of CB-si-BV2 cells in H2O2 group and (H2O2+GdCl3) group. In the microglial oxidative stress model, CatB plays a role in regulating the activation of NLRP3 inflammasome, which is probably mediated by calcium signaling.


Full-Text | PDF

Share this       

antalya escort

izmir rus escort

bursa escort bayan

antalya escort bayanlar

izmir escort

porno indir

porno izle

beşiktaş escort

eskişehir escort

burdur escort

bartın escort

havalandırma

türk takipçi satın al

smmabi.com

takipbonus.com

izmir escort

bursa escort

türk porno

escort bayan

yabanci porno

takipçi satın al

takipçi satın al

instagram takipçi satın al

instagram beğeni satın al

instagram takipçi satın al

takipçi satın alma

escort

panel smm

takipçi satın al instagram

smm panel

ataköy escort

izmit escort

Ledger Live desktop: Your secure gateway to crypto. Secure your assets with confidence using Ledger Live desktop. Experience a secure gateway that prioritizes the safety of your digital assets, providing peace of mind.