Abstract

Beta-aescinateImproved the Learning and Memory Abilities and Decreaedbeta4 Deposition through the Anti-Inflammatory Pathway in the Rat Amyloidal Model

Author(s): Huanmin Gao

β-aescinate has a wide variety of pharmacological properties, including anti-inflammatory, analgesic, and antipyretic activities. The inflammatory pathways in the brain have been closely related to pathological development of the Alzheimer’s Disease (AD). However, the anti-inflammatory mechanism of β-aescinate on AD has not been scrutinized. Thus the present study was designed to investigate whether β-aescinate improve the abilities such as learning and memory through the anti-inflammatory pathway in the rat amyloid model. Aβ1-40 was microdialyzed into the lateral ventricle. Learning and memory were evaluated by Morris Water Maze (MWM) test. Pathological changes in the hippocampus were estimated by H&E stained. The quantity of neuroglia and cytokine were investigated by immumohistochemistry. BSI-B4, GFAP, βA4 and TNF-α positive cells in Aβ group were higher than that in the sham group (P<0.01, respectively), but the latent period was lengthen in Aβ group compared with sham group significantly (P<0.05); BSI-B4, GFAP, βA4 and TNF-α positive cells were lower than that in Aβ+β-aescinate group significantly (P<0.01), and the latent period reduced in Aβ+β-Aescine group compared with Aβ+saline group significantly (P<0.01). These results suggested that β-aescinate decreased the activation of neuroglia, reduced inflammatory cytokines and decreased βA4 deposition, and improved the abilities of learning and memory through the anti-inflammatory pathway in the rat amyloidal mode