Research Article - (2018) Volume 8, Issue 5
Insomnia in Peri- and Postmenopausal Women: Plasma Lipids, Lipid Peroxidation and Some Antioxidant System Parameters
- Corresponding Author:
- Semenova Natalya V
Timiryasev str. 16, Irkutsk- 664003 Russia
According to the current data, most menopausal women have reduced quality of life and one of the main criteria is sleep. It is possible, that sleep loss may cause oxidative stress. This study determines sleep disorders characteristics, lipid profile, lipid peroxidation and antioxidant system parameters in peri- and postmenopausal women.
126 menopausal women divided into perimenopausal (n=56) and postmenopausal (n=70) groups were examined. Each group was divided into control (without sleep disorders) and main groups (insomnia and insomnia with obstructive sleep apnea syndrome (OSAS). Pittsburg Sleep Quality Index, Epworth Sleepiness Scale, Insomnia Severity Index, polysomnography were used for the assessment of sleep disorders. Lipid metabolism parameters by the enzymatic method were determined. Lipid peroxidation and antioxidant system parameters (conjugated dienes (CDs), malonyl dialdehyde (MDA), α-tocopherol, retinol, reduced and oxidized glutathione by spectrophotometric and fluorometric methods were determined.
The increase of total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) levels were observed in perimenopausal women with insomnia and OSAS. The increase of TC, triacylglycerol (TG), LDL-C, very-low-density lipoprotein cholesterol (VLDL-C) levels and decrease of high-density lipoprotein cholesterol (HDL-Ð¡) level were found in postmenopausal women with insomnia and OSAS as compared to control and group with insomnia. Accumulation of lipid peroxidation products was observed both in perimenopausal women with insomnia (CDs) and in postmenopausal women with insomnia (CDs, MDA) and insomnia with OSAS (MDA). There were no differences in antioxidant system parameters between main and control groups.
Both insomnia and insomnia with OSAS are associated with oxidative stress only in postmenopausal women. There are no differences in the accumulation of MDA between insomnia and insomnia with OSAS both in perimenopause and postmenopause.
Insomnia, Sleep apnea, Lipid metabolism, Lipid peroxidation, Antioxidant system, Perimenopause, Postmenopause
Menopause is one of the critical periods in a woman’s life and transition period from reproductive phase to its extinction and cessation of menstrual and genital function: follicular reserve of ovaries depletion, decreasing of its functional activity, changes in hormones relationship, estrogen level decreasing . 60%- 80% of menopausal women have vegetovascular, mental and metabolic disorders. Among the main signs of neuro-vegetative changes in menopausal women are sleep disorders. Some studies have shown that sleep problems are reported by 39%- 47% of perimenopausal women and 35%-60% of postmenopausal ones . According to the current data, most menopausal women have reduced life quality and one of the main criteria is sleep quality .
In recent years, it has been suggested that sleep has a regulating role in lipid homeostasis [4,5]. Moreover, it has been shown that the lipids concentration might depends on the circadian system . Also, experimental investigations has shown, that sleep loss may cause oxidative stress [7-12], which is a result of imbalance between free radicals production and antioxidant system activity . These can lead to development of different cardiovascular , endocrine , mental , oncology  and other pathologies. The consequences of lipid peroxidation products accumulation are changes in the metabolism of proteins, fats, carbohydrates, nucleic acids, water and electrolyte metabolism, which can cause severe tissue damages and organism adaptive capacity reduction . The main investigations in human are devoted to oxidative stress and obstructive sleep apnea syndrome association (OSAS) [18-31]. There are few investigations on insomnia and lipid peroxidation relationship [32-34], but it is obvious that this data are of great importance for elaborating of preventive and therapeutic measures to improve life quality of menopausal women.
The aim of this study is to determine lipid profile, lipid peroxidation and antioxidant system in peri- and postmenopausal women with insomnia.
Materials and Methods
The present study was conducted in the Scientific Centre for Family Health and Human Reproduction Problems (Irkutsk, Russia) with ethical standards of Helsinki Declaration (World Medical Association Declaration of Helsinki, 2008). All participants provided written, informed consent. The program of the study included the following methods: clinicalanamnestic (questionnaire, general medical examination, gynecological examination, polysomnographic monitoring (PSG), laboratory (lipid profile, lipid peroxidation, antioxidant system parameters examination) and statistical. 126 menopausal women divided into 2 groups: perimenopause (n=56) and postmenopause (n=70) were examined.
Inclusion criteria for perimenopausal group: Age 45-55; oligomenorrhoea or amenorrhea during last 12 months; ultrasounds criteria: 1) endometrial dysfunction: mismatch of structure and thickness corresponding to the first and the second phases of the menstrual cycle; 2) ovaries follicular reserve depletion.
Inclusion criteria for postmenopausal group: Age 56-60; amenorrhea ≥ 12 months; follicle stimulating hormone level >20 iU/ ml, index luteinizing hormone/folliclestimulating hormone <1; ultrasounds criteria: 1) thin non-functional endometrium, endometrial echo thinner than 5 mm; 2) lack of ovaries follicular reserve.
Exclusion criteria: exacerbation of chronic diseases; hormone replacement therapy; surgical menopause; the presence of chronic sleep disorders before menopause (insomnia, parasomnia, hypersomnia, obstructive sleep apnea syndrome); the use of hypnotic pills in the previous two weeks; shift work.
After questioning and PSG, the each group was divided into 3 subgroups: 2 main (insomnia; insomnia with OSAS) and control (without sleep disorders). 33.9% perimenopausal and 37.1% postmenopausal women had no sleep problems and formed control groups. Basic characteristics of women are shown in Table 1.
|control||insomnia||insomnia and OSAS||control||insomnia||insomnia and OSAS|
|Mean ± SD|
|age, years||49.08 ± 2.84||50.41 ± 3.43||50.61 ± 3.14||57.16 ± 1.12||58.02 ± 2.07||58.82 ± 2.21||P1-4*
|BMI, kg/m2||27.18 ± 4.58||29.11 ± 5.42||31.72 ± 5.59||27.96 ± 3.57||26.87 ± 3.28||33.81 ± 6.41||P1-3*
|Duration of insomnia (years)||-||1.45 ± 1.02||2.03 ± 1.32||-||5.34 ± 1.22||6.41 ± 1.21||P2-5*
Table 1: Subject profiles.
▪ Methods and elaborating on methods
The PSQI questionnaire consists of 19 items including subjective sleep quality, sleep latency, sleep duration, habitual sleep efficiency, sleep disturbances, use of sleeping medications, and daytime dysfunction. The total score of PSQI is 21 points.
The ISI questionnaire consists of 7 items. The answer each of the questions from 0 to 4 and a total score ranging from 0 (minimum) to 24 (maximum). The ISI results 0-7, 8-14, 15-21, 22- 28 points were interpreted as norm, slight sleep disorders, moderate and expressed respectively.
The ESS consists of 8 items. The answer each of the questions from 0 (not at all likely to fall asleep) to 3 (very likely to fall asleep) and a total score ranging from 0 (minimum) to 24 (maximum). The ESS results 0-3, 3-9, 9-16, 16- 24 points were interpreted as norm, insomnia, obstructive sleep apnea syndrome and narcolepsy respectively.
Polysomnographic monitoring: A full PSG was carried out with the using of GRASSTELEFACTOR Twin PSG (Comet) system with As 40 amplifier with an integrated module for sleep SPM-1 (USA) by standard method .
Collection of material: Venous blood sampled after 12-h of overnight fasting into tubes with EDTA between 8.00 and 9.00 a.m. Samples centrifuged for 10 min at 1.500 g at 4°C, and erythrocytes washed three times with 0.9% NaCl (wt/vol). Samples were used immediately (for total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and triacylglycerol (TG) determination) or kept frozen at -40°C, but not more than one month (for determination lipid peroxidation and antioxidant defense system parameters).
Plasma lipids determination: TC, HDL-C and TG levels were measured photometrically by LIVA-Lachema kits on a BTS-330 automatic analyzer (Poland). The low-density lipoprotein cholesterol (LDL-C) and very-low-density lipoprotein cholesterol (VLDL-C) levels in serum was estimated mathematically with using the Friedewald formula . The following lipid and lipoprotein levels were considered normal: TC <5.2 mmol/l, LDL-С <3.89 mmol/l, HDL-С <0.9 mmol/l, TG <1.7 mmol/l.
Lipid peroxidation products determination: Conjugated dienes (CDs) and end-product – malonyl dialdehyde (MDA) levels in blood plasma were evaluated with spectrofluorofotometer «Shimadzu RF-1501» (Japan). The CDs concentrations were detected by absorbance of plasma heptanes extracts at 232 nm . Coefficient of molar absorption (К=2.2˟105 М-1 С-1) was used for absorption units to μmol/L conversion.
MDA levels were determined by reaction with thiobarbituric acid followed fluorescence intensity at 515 nm (excitation) and 554 nm (emission) measurement . MDA concentration was expressed in μmol/L.
Antioxidant system parameters determination: Antioxidant system activity was detected by measurement of α-tocoferol, retinol levels in serum and reduced and oxidized glutathione (GSH, GSSG) levels in red cells using the spectrofluorofotometer «Shimadzu RF-1501» (Japan).
GSH and GSSG levels were detected in erythrocytes and performed at λ=350 nm (excitation) and λ=420 nm (absorption) . Concentrations were expressed in mmol/L.
Alfa-tocopherol and retinol levels were detected in the plasma . 0.2 mL of the plasma and 1 mL H2O was added in the samples. After shaking the samples 1 mL C2H5OH was added. After incubation for 15-20 min at 4°C, 5 mL C6H14 was added in the samples and was shaken for 45 min. Then the samples stayed for 20 min and measurement was performed at λ=294 nm (maximum excitation) and λ=330 nm (maximum radiation) for α-tocopherol and at λ=335 nm (maximum excitation) and λ=460 nm (maximum radiation) for retinol. Concentrations were expressed in mcmol/L.
▪ Statistical analysis
Statistical analysis was performed by STATISTICA 6.1 software (Stat-Soft Inc., USA) with parametric T-test (Student) and non-parametric U-test (Mann-Whitney) with significance level p<0,05. For descriptive analysis, results are presented as mean ± standard deviation (SD), median (inter-quartile range), 25th-75th percentile.
Questionnaire and PSG results are shown in Table 2. PSQI, ISI and ESS points were higher in main groups as compared to controls. Moreover, ESS points were higher in groups with OSAS as compared to insomnia. The decrease of sleep efficiency and the increase of sleep latency were found in main groups as compared to controls. The time of non-rapid eye movement sleep stage 3 was less in group with OSAS as compared to control.
|control||insomnia||insomnia and OSAS||control||insomnia||insomnia and OSAS|
|Mean ± SD|
|PSQI (points)||2.11 ± 0.53||16.21 ± 3.11||15.87 ± 2.63||3.08 ± 1.36||14.99 ± 1.98||16.12 ± 1.85||P1-2*
|ISI (points)||3.13 ± 0.97||22.8 ± 0.69||22.46+1.03||4.05 ± 2.11||25.2 ± 0.72||24.22+2.90||P1-2*
|ESS (points)||1.64 ± 0.33||6.63 ± 0.54||12.39 ± 2.44||2.08 ± 1.02||7.42 ± 1.88||15.31 ± 0.83||P1-2*
|Sleep efficiency (%)||96.21 ± 2.20||75.14+3.32||71.74 ± 8.43||92.51 ± 5.93||70.32 ± 3.42||68.41 ± 5.43||P1-2*
|Sleep latency (min)||19.28 ± 6.15||49.53 ± 2.11||38.10 ± 5.38||22.33 ± 18.16||36.69 ± 9.76||46.44 ± 6.12||P1-2*
|N1 (%)||5.52 ± 1.61||7.90 ± 3.15||51.15 ± 3.71||7.61 ± 4.17||10.10 ± 5.69||6.92 ± 2.82||-|
|N2 (%)||58.25 ± 15.90||53.51 ± 18.41||61.51 ± 15.94||53.95 ± 7.02||58.19 ± 19.24||64.24 ± 18.77||-|
|N3 (%)||23.20 ± 5.85||18.64 ± 5.78||11.10 ± 4.99||19.42 ± 3.85||15.94 ± 7.46||10.20 ± 6.10||P1-3*
|REM (%)||20.17 ± 5.65||21.72 ± 10.41||22.81 ± 12.14||25.89 ± 4.33||21.76 ± 14.15||20.65 ± 9.80||-|
|AHI (event / hour)||3.21 ± 0.53||4.82±1.50||13.27 ± 2.42||4.11 ± 0.15||5.51 ± 0.28||15.22 ± 3.48||P1-3*
|SaO2 (%)||96.45 ± 3.59||95.61 ± 0.28||90.11 ± 3.10||96.27 ± 2.12||93.36 ± 0.58||90.15 ± 4.58||-|
*- р < 0.05
Table 2: Questionnaire and polysomnographic monitoring results in menopausal women.
Table 3 summarized lipid profile in women with sleep disorders and control. TC and LDL-C levels were significant higher in perimenopausal women with insomnia and OSAS as compared to control and group with insomnia (p<0.05). Also, the increase of TC, TG, LDL-C, VLDL-C levels and decrease of HDL-С level were found in postmenopausal women with insomnia and OSAS as compared to control and group with insomnia (p<0.05).
|control||insomnia||insomnia and OSAS||control||insomnia||insomnia and OSAS|
|Mean ± SD
25th – 75th percentile
|4.38 ± 0.85
|4.51 ± 1.05
|5.23 ± 1.18
|5.36 ± 1.20
|5.06 ± 1.25
|6.37 ± 1.21
|0.93 ± 0.56
|0.92 ± 0.52
|0.98 ± 0.39
|1.12 ± 0.53
|1.07 ± 0.58
|1.63 ± 0.22
|1.26 ± 0.28
|1.25 ± 0.34
|1.18 ± 0.26
|1.16 ± 0.23
|1.23 ± 0.33
|0.91 ± 0.19
|2.64 ± 0.84
|2.85 ± 0.88
|3.61 ± 1.21
|3.69 ± 1.11
|3.36 ± 1.12
|4.78 ± 0.79
|0.42 ± 0.26
|0.42 ± 0.24
|0.45 ± 0.18
|0.51 ± 0.24
|0.49 ± 0.26
|0.69 ± 0.20
Table 3: Lipid profile parameters in menopausal women with sleep disorders and control.
Table 4 summarized lipid peroxidation and antioxidant system parameters in peri- and postmenopausal women. CDs levels were significant higher in group with insomnia as compared to control and group with insomnia and OSAS in perimenopause (р<0.05). CDs and MDA levels in insomnia group and MDA levels in group with insomnia and OSAS were significant higher as compared to control in postmenopause (р<0.05). No differences in α-tocopherol, retinol, GSH, GSSG between main groups and controls both perimenopause and postmenopause registered.
|control||insomnia||insomnia and OSA||control||insomnia||insomnia and OSA|
|Mean ± SD
25th – 75th percentile
|1.10 ± 0.35
|1.38 ± 0.89
|0.95 ± 0.60
|1.23 ± 0.70
|1.75 ± 0.69
|1.39 ± 0.81
|1.14 ± 0.52
|1.34 ± 0.52
|1.12 ± 0.32
|0.89 ± 0.27
|1.08 ± 0.33
|1.13 ± 0.45
|2.67 ± 0.53
|2.52 ± 0.48
|2.68 ± 0.37
|2.46 ± 0.45
|2.50 ± 0.54
|2.51 ± 0.48
|2.17 ± 0.60
|1.84 ± 0.41
|1.85 ± 0.53
|1.87 ± 0.35
|1.95 ± 0.48
|1.90 ± 0.50
|8.71 ± 2.56
|8.51 ± 4.00
|8.14 ± 2.43
|6.35 ± 1.42
|6.89 ± 3.00
|6.68 ± 1.55
|retinol (mkmol/l)||0.73 ± 0.19
|0.79 ± 0.31
|0.66 ± 0.23
|0.64 ± 0.18
|0.65 ± 0.19
|0.68 ± 0.19
Table 4: Lipid peroxidation–antioxidant system parameters in women with sleep disorders and control.
It has been proposed that cerebral free radicals accumulating during wakefulness and are removing during sleep. Removal of excess free radicals during sleep is accomplished by decreased rate of free radicals and increased efficiency of endogenous antioxidant mechanism . So sleep disorders may lead to the accumulation of free radicals in the body.
The results of the experimental studies insomnia and oxidative stress association are ambiguous. Thus, sleep loss may be oxidative damage cause of the brain, because it is sensitive to oxidative stress due to polyunsaturated fatty acids high content in the brain cells membranes and low antioxidant capacity . But other study have not shown any changes in rats brain, liver, skeletal muscle proteins free radical oxidation and lipids parameters at 1-2 week of sleep deprivation . Moreover, lipid peroxidation parameters association with sleep deprivation time has been found. Thus, it has been shown an increase of MDA blood plasma level at 48 h and 72 h, while minimal change at 24 h and 96 h of sleep deprivation period . Also, there are differences in antioxidant defense system parameters depending on the sleep deprivation period. Thus, at short-term total sleep deprivation (6 h) there is increase GSH level in the cortex, brain stem and basal forebrain, and glutathione peroxidase activity in the hippocampus and cerebellum , and decrease Cu/Zn-superoxide dismutase activity in the hippocampus and brain stem at 5-10 days sleep deprivation .
Some studies have reduced glutathione peroxidase activity and increased MDA levels have shown in human insomnia, whereas myeloperoxidase and superoxide dismutase activity and glutathione level not changed . Patients with insomnia have reduced total antioxidant status and increased systemic oxidative stress. Authors suggested that decreased of paraoksanase activity may play role in the development of oxidative stress in these patients . However, main groups of these studies include both men and women, and gender influence was not considered, although gender differences in lipid peroxidation – antioxidant defense system are evident . Zhao et al. study have demonstrated lower serum uric acid levels in patients with chronic insomnia that can lead to oxidative stress because uric acid is considered as a major antioxidant. Moreover, among chronic insomnia patients, decreased serum uric acid levels were associated with poor sleep quality .
In our investigation both insomnia and insomnia with OSAS with the development of oxidative stress and accumulation of lipid peroxidation products was found only in postmenopausal women that can be connected with duration of sleep disorders and mild OSAS in perimenopausal women. However, changes in antioxidant defense system parameters were not found. Our findings are consistent with Hachul et al. who also demonstrated an increased thiobarbituric acid reactants level and similar with control catalase and superoxide dismutase activities in postmenopausal women with insomnia .
It is possible that sleep has a regulating role in lipid homeostasis [4,5]. The results of study in Chinese population have shown the decrease in choline plasmalogen levels demonstrating that these lipids are susceptible to degradation by oxidative stress and the increase in phosphatidylcholines and triacylglycerides in men with sleep deprivation . Another study has not shown a lipid metabolism changes and insomnia association in men. However, dyslipidemia and insomnia association with the increase in total cholesterol levels has been found in menopausal women with frequent insomnia. One of the mechanisms proposed to explain the insomnia and dyslipidemia link might be the increase activity of hypothalamicpituitaryadrenal- axis and excessively cortisol secretion . Increased midnight cortisol levels in menopausal women with primary chronic insomnia have been demonstrated in Seeling et al. study. However, it was not associated with impaired metabolism of lipids , which agrees with our data.
It is known that hypoxia during OSAS is a stress factor which may change free radical homeostasis, manifested in reactive oxygen products deficiency and activation of lipid peroxidation [49,50]. Thus, an increasing of free radicals level in leukocytes , plasma, urine and condensate of exhaled air , intensification of lipid peroxidation processes [19,25,26], oxidation of DNA  have been demonstrated in patients with OSAS. However, some studies have not shown assosiation of oxidative stress with OSAS [21-23]. This leaves the question of its association with this pathology. The results of our study demonstrated no differences in lipid peroxidation and GSH, GSSG, retinol, α- tocopherol levels in women with insomnia and OSAS as compared to women only with insomnia. Simiakakis et al. in their study has found that obesity and smoking have a greater impact on the development of oxidative stress than hypoxia, whose role is to enhance the already existing oxidative stress .
Moreover, reduced antioxidant activity in patients with OSAS without influence obesity and smoking has been shown. It may be due to the genes disregulation involved in the modulation of reactive oxygen species or enzymatic antioxidants. A decrease of total antioxidant activity in patients with OSAS has been also demonstrated in some studies [25,51], however, there were no changes in the superoxide dismutase  and glutathione peroxidase activity . Moreover, it has been shown that oxidative stress intensity correlates with the degree of OSAS severity [24,28,30]. High frequency of hypoxia episodes leads to more pronounced oxidative stress and oxidation of DNA involving anti-aging genes. Down regulation of anti-aging genes reduces hepatic xenobiotic metabolism that lead to involvement toxic compounds in nuclear receptor dysfunction such as the nuclear receptor Sirtuin 1 . Sirtuin 1 involves in epigenetic processes and its defects lead to defective xenobiotic metabolism with effects on mitophagy and corruption of the nuclear-mitochondria interaction. It has been shown Sirtuin 1 regulates the suprachiasmatic nucleus in the hypothalamus with effects on melatonin secretion .
Melatonin plays an important role in activation of antioxidant system enzymes, such as superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glucose-6-phosphate dehydrogenase . So, melatonin deficiency might contribute to the development of oxidative stress. It is well known that melatonin hormone is one of the key regulators of circadian biorhythms and violation of its secretion in menopausal women might lead to changes in metabolism and the formation of various diseases, including sleep disturbances. Studies carried out to date have been shown that people with insomnia have a lower melatonin level ; moreover, the peak of the hormone secretion is shifted, which have been demonstrated in the study on the association of melatonin, menopausal depression and sleep time. A delay in the peak of hormone secretion before morning have been revealed in women with menopausal depression, which, according to researchers, may be caused by longer sleep duration as a compensation for insomnia in these women . Similar results have been obtained in our study in which the shift in the peak of melatonin secretion in the early morning hours in women with insomnia in perimenopause was shown. In postmenopause, the morning melatonin peak has been also noted, regardless of the presence of insomnia . The present study further have revealed the association of melatonin secretion circadian rhythms with Clock 3111T/C gene polymorphism in Caucasian patients with insomnia, consisting of an increased hormone level in early morning hours in carriers of the TT-genotype, which allows considering 3111T allele as risky in the formation of melatonin circadian rhythm disturbances in these patients . Moreover, differences in the circadian rhythms of melatonin in the Asian women depending on the genotype of the Clock 3111T/C polymorphism have not been found. Is there circadian rhythm melatonin and oxidative stress relationship in women of different ethnic groups with insomnia is unclear. The results of some studies have demonstrated links between the circadian clock and Sirtuin 1 function. It is possible that Sirtuin 1 defects lead to suprachiasmatic nucleus in the hypothalamus defects are related to the peripheral circadian clock dyssynchrony [52,59]. Probably, oxidative stress severity in Asian women with insomnia will differed from Caucasian ones. However, to proof this hypothesis, further researches are required.
It has been shown that insomnia leads to memory impairment and memory depends on the subtype insomnia . Also, close relationship of insomnia and mental diseases , obesity , cardiovascular diseases , diabetes , oncology  in whose pathogenesis there is role of oxidative stress has been significant proved. So, lipid peroxidation investigations in patients with sleep disorders deserve special attention for development of preventive and curative measures to improve health and quality of life.
It is well known that xenobiotic biotransformation is reduced with aging. Xenobiotics induce epigenetic changes that involve chromatin remodelling by alterations in transcriptional regulators with modification of histones that lead to metabolic disorders with neurodegeneration. Sirtuin 1 defects lead to suprachiasmatic nucleus in the hypothalamus defects and changes of melatonin level . Firstly, melatonin is one of the key regulators of circadian biorhythms and violation of its secretion might lead to the formation of insomnia with cerebral free radicals accumulation . Secondly, melatonin plays an important role in antioxidant system and its deficiency might contribute to the development of oxidative stress .
Our data proved that both insomnia and insomnia comorbidity with OSAS are associated with oxidative stress only in postmenopausal women. It is possible that the duration of sleep disorders influences the accumulation lipid peroxidation products. There are no differences in the accumulation of MDA between insomnia and insomnia with OSAS that can be connected with mild OSAS in perimenopausal women and moderate OSAS in postmenopausal ones.
Conflict of Interest
The authors state no conflict of interest.
- O'Neill S, Eden J. The pathophysiology of menopausal symptoms. Obstet. Gynecol. Reprod. Med 24(12), 349-356 (2014).
- Xu Q, Lang CP. Examining the relationship between subjective sleep disturbance and menopause: a systematic review and meta-analysis. Menopause 21(12), 1301-1318 (2014).
- Lo CMH, Lee PH. Prevalence and impacts of poor sleep on quality of life and associated factors of good sleepers in a sample of older Chinese adults. Health. Qual. Life. Outcomes 72(1), (2012).
- Davies SK, Ang JE, Revell VL, et al. Effect of sleep deprivation on the human metabolome. Proc. Nat. Acad. Sci. USA 111(29), 10761–10766 (2014).
- Weljie AM, Meerlo P, Goel N, et al. Oxalic acid and diacylglycerol 36:3 are cross-species markers of sleep debt. Proc. Nat. Acad. Sci. USA 112(8), 2569–2574 (2015).
- Chua EC-P, Shui G, Cazenave-Gassiot A, et al. Changes in plasma lipids during exposure to total sleep deprivation. Sleep 38 (11), 1683–1691 (2015).
- Ramanathan L, Gulyani CS, Nienhuis R, et al. Sleep deprivation decreases superoxide dismutase activity in rat hippocampus and brainstem. Neuroreport 13(11), 1387-90 (2002).
- Ramanathanl L, Hu S, Frautschy SA, et al. Short-term total sleep deprivation in the rat increases antioxidant responses in multiple brain regions without impairing spontaneous alternation behavior. Behav. Brain. Res 207(2), 305-309 (2010).
- Gopalakrishnan A, Cirelli C. Sleep deprivation and cellular responses to oxidative stress. Sleep 27(1), 27-35 (2004).
- Suer C, Dolu N, Artis AS, et al. The effects of long-term sleep deprivationon on the long-term potentiation in the dentate gyrus and brain oxidation status in rats. Neurosci. Res 70, 71-77 (2011).
- Thamaraiselvi K, Mathangi DC, Subhashini AS. Effect of increase in duration of REM sleep deprivation on lipid peroxidation. Int. J. Biol. Med. Res 3(2), 1754-1759 (2012).
- MathangiDC, Shyamala R, SubhashiniAS. Effect of REM sleep deprivation on the antioxidant status in the brain of wistar rats. Ann. Neurosci 19 (4), 161–164 (2012).
- Sies H. Oxidative stress: a concept in redox biology and medicine. Redox. Biol 4(1), 180-183 (2015).
- Sinha N, Dabla PK. Oxidative stress and antioxidants in hypertension-a current review. Curr. Hypertens. Rev 11(2), 132-142 (2015).
- Kolesnikova LI, Darenskaya MA, Vlasov BYa, et al. Oxidative stress in Mongoloids and Caucasians with type 1 diabetes. J. Diabetes. Met 5,376 (2014).
- Morris G, Stubbs B, Köhler CA, et al. The putative role of oxidative stress and inflammation in the pathophysiology of sleep dysfunction across neuropsychiatric disorders: Focus on chronic fatigue syndrome, bipolar disorder and multiple sclerosis. Sleep. Med. Rev S1087-0792(17), 30152-30161 (2018).
- Lee WL, Huang JY, Shyur LF. Phytoagents for Cancer Management: Regulation of Nucleic Acid Oxidation, ROS, and Related Mechanisms. Oxid. Med. Cell. Longev 925804, 1-22 (2013).
- Dyugovskaya L, Lavie P, Lavie L. Increased adhesion molecules expression and production of reactive oxygen species in leukocytes of sleep apnea patients. Am. J. Respir. Crit. Care. Med 165(7), 934–939 (2002).
- Vishnevsky A, Lavie P. Evidence for lipid peroxidation in obstructive sleep apnea. Sleep 27 (1), 123–128 (2004).
- Yamauchi M, Nakano H, Maekawa J, et al. Oxidative stress in obstructive sleep apnea. Chest 127(5), 1674–1679 (2005).
- Oztürk L, Mansour B, Yüksel M, et al. Lipid peroxidation and osmotic fragility of red blood cells in sleep apnea-patients. Clinica. Chimica. Acta 332(1–2), 83–88 (2003).
- Alzoghaibi MA, Bahammam AS. Lipid peroxides, superoxide dismutase and circulating IL-8 and GCP-2 in patients with severe obstructive sleep apnea: a pilot study. Sleep. Breath 9(3), 119–126 (2005).
- Svatikova A, Wolk R, Lerman LO, et al. Oxidative stress in obstructive sleep apnoea. Eur. Heart. J 26(22), 2435–2439 (2005).
- Lloret A, Buj J, Badia MC, et al. Obstructive sleep apnea: arterial oxygen desaturation coincides with increases in systemic oxidative stress markers measured with continuous monitoring. Free. Radic. Biol. Med 42(6), 893–894 (2007).
- Wysocka E, Cofta S, Cymerys M, et al. The impact of the sleep apnea syndrome on oxidant-antioxidant balance in the blood of overweight and obese patients. J. Physiol. Pharmacol 59(6), 761–769 (2008).
- Baysal E, Taysi S, Aksoy N, et al. Serum paraoxonase, arylesteraseactivity and oxidative status in patients with obstructive sleep apnea syndrome (OSAS). Eur. Rev. Med. Pharmacol. Sci 16, 770-774 (2012).
- Lavie L. Oxidative stress inflammation and endothelial dysfunction in obstructive sleep apnea. Front. Biosci. (Elite Ed) 4:1391-1403 (2012).
- Franco CM, Lima AM, Ataide LJr, et al. Obstructive sleep apnea severity correlates with cellular and plasma oxidative stress parameters and affective symptoms. J. Mol. Neurosci 47(2), 300-310 (2012).
- Simiakakis M, Kapsimalis F, Chaligiannis E, et al. Lack of effect of sleep apnea on oxidative stress in obstructive sleep apnea syndrome (OSAS) patients. PLoSOne 7(6), e39172 (2012).
- Hopps E, Canino B, Calandrino V, et al. Lipid peroxidation and protein oxidation are related to the severity of OSAS. Eur. Rev. Med. Pharmacol. Sci 18(24), 3773-3778 (2014).
- Passali D, Corallo G, Yaremchuk S, et al. Oxidative stress in patients with obstructive sleep apnoea syndrome. Acta Otorhinolyaryngol. Ital 35(6), 420-425 (2015).
- Hachul DE, Campos H, Brandao LC, et al. Sleep disturbances, oxidative stress and cardiovascularrisk parameters in postmenopausal women complaining of insomnia. Climacteric 9(1), 312-319 (2006).
- Gulec M, Ozkol H, Selvi Y, et al. Oxidative stress in patients with primary insomnia. Prog. Neuropsychopharmacol. Biol. Rsychiatry 37(2), 247-251 (2012).
- Liang B, Li Y-H, Kong H. Serum paraoxonase, arylesterase activities and oxidative status in patients with insomnia. Eur. Rev. Med. Pharmacol. Sci 17(1), 2517-2522 (2013).
- Buysse D, Reynolds C, Monk T, et al. The Pittsburgh Sleep Qulity Index: a new instrument for psychiatric practice and research. Psychiatry. Res 28(1), 193-213 (1989).
- Savard J, Simard S, Morin C. Insomnia / In A. Nikcevic A. Kuczmierczyk, M. Bruch (Edtn) Formulation and treatment in clinical health psychology Routledge, London (2006).
- Johns MW. New method for measuring daytime sleepiness: the Epworth sleepiness scale. Sleep 14(1), 540-545 (1991).
- Rechtschaffen A, Kales A. Manual of standardized terminology, tecniques, and criteria for the scoring of stages of sleep and wakefulness of human subjects. Washington, DL (1968).
- Friedewald WT, Levy RI, Fredrickson DS. Estimation of the Concentration of Low-Density Lipoprotein Colуsterol in Plasma, Without Use of the Preparative Ultracentrifuge. Clin. Chemistry 18(6), 499-502 (1972).
- Corongiu FP, Banni S. Detection of conjugated dienes by second derivative ultraviolet spectrophotometry. Methods. Enzymol 23(3), 303-310 (1994).
- Yagy KA. Assay for serum lipid peroxide level and its clinical significance. In Yagi K. (ed.) Lipid Peroxides in Biology and Medicine, 223-242 (1982).
- Hisin PJ, Hilf R. Fluorоmetric method for determination of oxidized and reduced glutathione in tissues. Anal. Biochem 74(1), 214–226 (1976).
- Cherniauskene RCh, Varshkiavichene ZZ, Gribauskas PS. Simultaneous fluorometric determination of the concentrations of vitamins E and A in blood serum. Lab. Delo 6(1), 362–365 (1984).
- Reimund E. The free radical flux theory of sleep. Med. Hypotheses 43(4), 231-233 (1994).
- Mendoza-Nunez VM, Beristain-Perez A, Perez-Vera SP, et al. Age-related sex differences in glutathione peroxidase and oxidative DNA damage in a healthy Mexican population. J. Womens. Health 19(5), 919-926 (2010).
- Zhao K, Luan X, Liu Z, et al. Low serum uric acid levels in chronic insomnia patients: A case-control study. Neurosci. Lett 657(1), 102-105 (2017).
- Zhan Y, Zhang F, Lu L, et al. Prevalence of dyslipidemia and its association with insomnia in a community based population in China. BMC. Public. Health 14(1), 1050 (2014).
- Seelig E, Keller U, Klarhofer M, et al. Neuroendocrine regulation and metabolism of glucose and lipids in primary chronic insomnia: a prospective case-control study. PLoSOne 8(4), e61780 (2013).
- Sasaki T, Awaji T, Shimada K, et al. Increased Levels of Reactive Oxygen Species in Brain Slices after Transient Hypoxia Induced By a Reduced Oxygen Supply. Neuropsychiatry 8(2), 684-690 (2018).
- Lin K-H, Lin H-Z, Lin Y-P, et al. Obstructive sleep apnea and retinal microvascular characteristics: a brief review. Neuropsychiatry 7(1), 12-21 (2017).
- Barcelo A, Barbe F, de la Pena M, et al. Antioxidant status in patients with sleep apnoea and impact of continuous positive airway pressure treatment. Eur Respir. J 27(4), 756–760 (2006).
- Martins IJ. Single Gene Inactivation with Implications to Diabetes and Multiple Organ Dysfunction Syndrome. J. Clin. Epigenet 3(3), 24 (2017).
- Martins IJ. Regulation of Core Body Temperature and the Immune System Determines Species Longevity. Curr. Updates. Gerontol 1(6.1), 1-6 (2017).
- Tan DX, Manchester LC, Terron MP, et al. One molecule, many derivatives: a never-ending interaction of melatonin with reactive oxygen and nitrogen species? J. Pineal. Res 42(1), 28-42 (2007).
- Xie Z, Chen F, Li WA, et al. A review of sleep disorders and melatonin. Neurological. Res 39(6), 559–565 (2017).
- Parry B, Meliska C, Sorenson D, et al. Increased melatonin and delayed offset in menopausal depression: Role of years past menopause, follicle-stimulating hormone, sleep end time, and body mass index. J. Clin. End. Metab 93(1), 54-60 (2008).
- Kolesnikova LI, Madaeva IM, Semenova NV, et al. Pathogenic role of melatonin in sleep disorders in menopausal women. Bull. Exp. Biol. Med 156(1), 104-106 (2013).
- Semenova NV, Madaeva IM, Bairova TA, et al. Association of the melatonin circadian rhythms with clock 3111T/C gene polymorphism in Caucasian and Asian menopausal women with insomnia. Chronobiol. Int 35 (2018).
- Martins IJ. Induction of NAFLD with Increased Risk of Obesity and Chronic Diseases in Developed Countries. Open. J. Endocr. Metab. Dis 4(4), 90-110 (2014).
- Xia L, Chen G-H, Wei Q-G, et al. Discrepancies of Cognition Among Different Subtypes and Correlations with Sleep Parameters in the Patients with Chronic Insomnia Disorder. Neuropsychiatry8(2), 570-579 (2018).
- Sivertsen B, Lallukka T, Salo P, et al. Insomnia as a risk factor for ill health: results from the large population-based prospective HUNT Study in Norway. J. Sleep. Res 23(2), 124-132 (2014).
- Patel SR, Blackwell T, Redline S, et al. The association between sleep duration and obesity in older adults. Int. J. Obes 32(12), 1825-1834 (2008).
- Tobaldini E, Costantino G, Solbiati M, et al. Sleep, sleep deprivation, autonomic nervous system and cardiovascular diseases. Neurosci. Biobehav. Rev 74(Pt B), 321-329 (2017).
- Xi B, He D, Zhang M, et al. Short sleep duration predicts risk of metabolic syndrom: a systematic review and meta-analysis. Sleep Med. Rev 18, 293-297 (2014).
- Savard J, Morin CM. Insomnia in the context of cancer: a review of a neglected problem. J. Clin. Oncol 1(1), 895-908 (2001).
- Martins IJ. Increased Risk for Obesity and Diabetes with Neurodegeneration in Developing Countries : Chapter1(2018).